Review



pcr amplification instrument  (Eppendorf AG)


Bioz Verified Symbol Eppendorf AG is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Eppendorf AG pcr amplification instrument
    Pcr Amplification Instrument, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 199205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+amplification/Eppendorf/pm42118147-289-6-9
    Average 99 stars, based on 199205 article reviews
    pcr amplification instrument - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Compositions and methods for enzyme immobilization
    Article Snippet: .. The success of these knockouts was determined by PCR amplification (Eppendorf Mastercycler 5333) and morphological characterization using the Laxco LMC4000 microscope (40× Objective, brightfield and fluorescent LED light sources) in conjunction with the Zeiss Sigma VP HD field SEM (UVA Advanced Microscopy Core). ..

    Article Title: Prevalence and molecular characterization of ESBL-producing Enterobacterales in clinical samples from the Boucle du Mouhoun region in Burkina Faso.
    Article Snippet: .. The PCR conditions were as follows: initial activation at 95°C for 5 min, followed by 30 cycles of denaturation at 95°C for 40 sec, annealing at 48°C for 40 sec (CTX-M-1 group) and 52°C for 40 sec (CTX-M-9 group), extension at 72°C for 1 min, and a final extension cycle at 72°C for 5 min. PCR amplification of the target genes was carried out using a Mastercycler® Nexus X2 thermal cycler (Eppendorf®, Hamburg, Germany). .. PCR products were visualized after electrophoresis on a 1% agarose gel containing ethidium bromide at 135 V for 45 min. A 100 bp DNA ladder (Promega, USA) was used as a size marker.

    Article Title: A metagenomic assessment of overburden dump of coal mine soil bacterial consortium from Jharkhand, India.
    Article Snippet: Overburden dump (OBD) of coal mine waste soils is arguably a major source of heavy metals and metalloids, leading to both public health and ecological consequences.. This study employed hypervariable V3–V4 region of 1.5 kbp 16S rDNA gene– based amplicon metagenomic sequencing to analyze unexplored bacterial diversity and its phenotypical and hypothetical functions, emphasizing the significance of these studies for assessing the potential of bioremediation.. Triplicate coal OBD soil samples were collected from the coal dumping yard of Sarubera Colliery from Atna-Chainpur and agricultural soils from Ramgarh Cantonment, Jharkhand.

    Article Title: Zinc sulfate acts as an efflux pump inhibitor on Pseudomonas aeruginosa clinical isolates
    Article Snippet: .. The PCR amplification was carried out in 50 μl reactions as follows: in Eppendorf 25 μl COSMO PCR Hot Start RED Master Mix, 5 μl of extracted DNA, and 1 M of each primer. ..

    Article Title: Insight into the efficient transmission of cucurbit aphid-borne yellows virus (Polerovirus CABYV) by Aphis gossypii Glover in bitter gourd
    Article Snippet: The Polerovirus cucurbit aphid-borne yellows virus (CABYV) infecting bitter gourd was efficiently transmitted, manifesting in symptoms like leaf cupping, complete yellowing, chlorotic patches, and orange discoloration of young leaves, accompanied by vein thickening.. Older leaves exhibited dark green mottling, yellowing along the margins, and a leathery texture.. In field conditions, the aphid population (8.95 per plant) and CABYV incidence (85.6 %) were the highest in Coimbatore district of Tamil Nadu, while the greatest yield loss of up to 72.38 % occurred in Erode district.

    Article Title: Compositions and methods for scalable production and delivery of biologicals
    Article Snippet: .. The success of these knockouts was determined by PCR amplification (Eppendorf Mastercycler 5333) and morphological characterization using the Laxco LMC4000 microscope (40× Objective, brightfield and fluorescent LED light sources) in conjunction with the Zeiss Sigma VP HD field SEM (UVA Advanced Microscopy Core). ..

    Article Title: Belowground perspectives: how plant functional type clipping reshapes soil fungal communities across peat depths
    Article Snippet: Vascular plant encroachment at the expense of Sphagnum mosses may threaten peatland carbon (C) stocks, yet the role of plant functional types (PFTs) and their fungal partners remains unclear.. We conducted an in situ clipping experiment in a montane peatland to examine how shrubs, graminoids, and Sphagnum mosses shape fungal abundance, diversity, and functional composition across acrotelm (0–20 cm) and mesotelm (20–30 cm) layers.. Shrub clipping reduced overall fungal diversity and the relative abundances of ericoid (ErMF) and ectomycorrhizal fungi (EcMF) in the acrotelm, while increasing the relative abundance of lignocellulosedegrading fungi.

    Article Title: makeTCR: A Modular Platform for Rapid, Scalable, Single-Step T Cell Receptor Synthesis
    Article Snippet: Two orthogonal primers were dispensed into each well at a final concentration of 0.2 μM, directly from 100 μM 96-well stock plates, using a 96-well plate based non-contact liquid handler (Dispendix I.DOT). .. Following reaction setup, plates were centrifuged at 1,000 rcf for 1 min and subjected to orbital shaking on a Bioshake iQ at 3,000 rpm for 3 min. Plates were centrifuged again prior to PCR amplification in an Eppendorf x50t under the following modified conditions: an initial three-step touchdown protocol starting at 65°C was implemented to enhance amplification specificity, followed by 33 cycles of PCR with an annealing temperature of 62°C and an extended annealing time of 40 sec to promote specific priming and efficient on-target amplification despite elevated annealing temperature. .. Post-PCR cleanup was performed using AMPure XP beads, dispensed at a 1:1 bead-to-reaction volume ratio using the Formulatrix Mantis.

    Amplification:

    Article Title: Compositions and methods for enzyme immobilization
    Article Snippet: .. The success of these knockouts was determined by PCR amplification (Eppendorf Mastercycler 5333) and morphological characterization using the Laxco LMC4000 microscope (40× Objective, brightfield and fluorescent LED light sources) in conjunction with the Zeiss Sigma VP HD field SEM (UVA Advanced Microscopy Core). ..

    Article Title: Prevalence and molecular characterization of ESBL-producing Enterobacterales in clinical samples from the Boucle du Mouhoun region in Burkina Faso.
    Article Snippet: .. The PCR conditions were as follows: initial activation at 95°C for 5 min, followed by 30 cycles of denaturation at 95°C for 40 sec, annealing at 48°C for 40 sec (CTX-M-1 group) and 52°C for 40 sec (CTX-M-9 group), extension at 72°C for 1 min, and a final extension cycle at 72°C for 5 min. PCR amplification of the target genes was carried out using a Mastercycler® Nexus X2 thermal cycler (Eppendorf®, Hamburg, Germany). .. PCR products were visualized after electrophoresis on a 1% agarose gel containing ethidium bromide at 135 V for 45 min. A 100 bp DNA ladder (Promega, USA) was used as a size marker.

    Article Title: A metagenomic assessment of overburden dump of coal mine soil bacterial consortium from Jharkhand, India.
    Article Snippet: Overburden dump (OBD) of coal mine waste soils is arguably a major source of heavy metals and metalloids, leading to both public health and ecological consequences.. This study employed hypervariable V3–V4 region of 1.5 kbp 16S rDNA gene– based amplicon metagenomic sequencing to analyze unexplored bacterial diversity and its phenotypical and hypothetical functions, emphasizing the significance of these studies for assessing the potential of bioremediation.. Triplicate coal OBD soil samples were collected from the coal dumping yard of Sarubera Colliery from Atna-Chainpur and agricultural soils from Ramgarh Cantonment, Jharkhand.

    Article Title: Zinc sulfate acts as an efflux pump inhibitor on Pseudomonas aeruginosa clinical isolates
    Article Snippet: .. The PCR amplification was carried out in 50 μl reactions as follows: in Eppendorf 25 μl COSMO PCR Hot Start RED Master Mix, 5 μl of extracted DNA, and 1 M of each primer. ..

    Article Title: Insight into the efficient transmission of cucurbit aphid-borne yellows virus (Polerovirus CABYV) by Aphis gossypii Glover in bitter gourd
    Article Snippet: The Polerovirus cucurbit aphid-borne yellows virus (CABYV) infecting bitter gourd was efficiently transmitted, manifesting in symptoms like leaf cupping, complete yellowing, chlorotic patches, and orange discoloration of young leaves, accompanied by vein thickening.. Older leaves exhibited dark green mottling, yellowing along the margins, and a leathery texture.. In field conditions, the aphid population (8.95 per plant) and CABYV incidence (85.6 %) were the highest in Coimbatore district of Tamil Nadu, while the greatest yield loss of up to 72.38 % occurred in Erode district.

    Article Title: Compositions and methods for scalable production and delivery of biologicals
    Article Snippet: .. The success of these knockouts was determined by PCR amplification (Eppendorf Mastercycler 5333) and morphological characterization using the Laxco LMC4000 microscope (40× Objective, brightfield and fluorescent LED light sources) in conjunction with the Zeiss Sigma VP HD field SEM (UVA Advanced Microscopy Core). ..

    Article Title: Belowground perspectives: how plant functional type clipping reshapes soil fungal communities across peat depths
    Article Snippet: Vascular plant encroachment at the expense of Sphagnum mosses may threaten peatland carbon (C) stocks, yet the role of plant functional types (PFTs) and their fungal partners remains unclear.. We conducted an in situ clipping experiment in a montane peatland to examine how shrubs, graminoids, and Sphagnum mosses shape fungal abundance, diversity, and functional composition across acrotelm (0–20 cm) and mesotelm (20–30 cm) layers.. Shrub clipping reduced overall fungal diversity and the relative abundances of ericoid (ErMF) and ectomycorrhizal fungi (EcMF) in the acrotelm, while increasing the relative abundance of lignocellulosedegrading fungi.

    Article Title: makeTCR: A Modular Platform for Rapid, Scalable, Single-Step T Cell Receptor Synthesis
    Article Snippet: Two orthogonal primers were dispensed into each well at a final concentration of 0.2 μM, directly from 100 μM 96-well stock plates, using a 96-well plate based non-contact liquid handler (Dispendix I.DOT). .. Following reaction setup, plates were centrifuged at 1,000 rcf for 1 min and subjected to orbital shaking on a Bioshake iQ at 3,000 rpm for 3 min. Plates were centrifuged again prior to PCR amplification in an Eppendorf x50t under the following modified conditions: an initial three-step touchdown protocol starting at 65°C was implemented to enhance amplification specificity, followed by 33 cycles of PCR with an annealing temperature of 62°C and an extended annealing time of 40 sec to promote specific priming and efficient on-target amplification despite elevated annealing temperature. .. Post-PCR cleanup was performed using AMPure XP beads, dispensed at a 1:1 bead-to-reaction volume ratio using the Formulatrix Mantis.

    Microscopy:

    Article Title: Compositions and methods for enzyme immobilization
    Article Snippet: .. The success of these knockouts was determined by PCR amplification (Eppendorf Mastercycler 5333) and morphological characterization using the Laxco LMC4000 microscope (40× Objective, brightfield and fluorescent LED light sources) in conjunction with the Zeiss Sigma VP HD field SEM (UVA Advanced Microscopy Core). ..

    Article Title: Compositions and methods for scalable production and delivery of biologicals
    Article Snippet: .. The success of these knockouts was determined by PCR amplification (Eppendorf Mastercycler 5333) and morphological characterization using the Laxco LMC4000 microscope (40× Objective, brightfield and fluorescent LED light sources) in conjunction with the Zeiss Sigma VP HD field SEM (UVA Advanced Microscopy Core). ..

    Activation Assay:

    Article Title: Prevalence and molecular characterization of ESBL-producing Enterobacterales in clinical samples from the Boucle du Mouhoun region in Burkina Faso.
    Article Snippet: .. The PCR conditions were as follows: initial activation at 95°C for 5 min, followed by 30 cycles of denaturation at 95°C for 40 sec, annealing at 48°C for 40 sec (CTX-M-1 group) and 52°C for 40 sec (CTX-M-9 group), extension at 72°C for 1 min, and a final extension cycle at 72°C for 5 min. PCR amplification of the target genes was carried out using a Mastercycler® Nexus X2 thermal cycler (Eppendorf®, Hamburg, Germany). .. PCR products were visualized after electrophoresis on a 1% agarose gel containing ethidium bromide at 135 V for 45 min. A 100 bp DNA ladder (Promega, USA) was used as a size marker.

    Modification:

    Article Title: makeTCR: A Modular Platform for Rapid, Scalable, Single-Step T Cell Receptor Synthesis
    Article Snippet: Two orthogonal primers were dispensed into each well at a final concentration of 0.2 μM, directly from 100 μM 96-well stock plates, using a 96-well plate based non-contact liquid handler (Dispendix I.DOT). .. Following reaction setup, plates were centrifuged at 1,000 rcf for 1 min and subjected to orbital shaking on a Bioshake iQ at 3,000 rpm for 3 min. Plates were centrifuged again prior to PCR amplification in an Eppendorf x50t under the following modified conditions: an initial three-step touchdown protocol starting at 65°C was implemented to enhance amplification specificity, followed by 33 cycles of PCR with an annealing temperature of 62°C and an extended annealing time of 40 sec to promote specific priming and efficient on-target amplification despite elevated annealing temperature. .. Post-PCR cleanup was performed using AMPure XP beads, dispensed at a 1:1 bead-to-reaction volume ratio using the Formulatrix Mantis.



    Similar Products

    99
    New England Biolabs pcr amplifications
    Pcr Amplifications, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+amplification/Q5+High-Fidelity+DNA+Polymerase/pmc12996797-76-1-10
    Average 99 stars, based on 1 article reviews
    pcr amplifications - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    98
    TaKaRa pcr amplification
    Pcr Amplification, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+amplification/PrimeSTAR+GXL+DNA+Polymerase/us12630855-244-10-16
    Average 98 stars, based on 1 article reviews
    pcr amplification - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    86
    Macrogen pcr amplification
    Pcr Amplification, supplied by Macrogen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+amplification/sanger+sequencing/pm42310464-778-11-9
    Average 86 stars, based on 1 article reviews
    pcr amplification - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Sangon Biotech pcr amplification
    Pcr Amplification, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+amplification/amplification/pmc13049309-196-1-15
    Average 86 stars, based on 1 article reviews
    pcr amplification - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Amoy Diagnostics human brafv600e amplification refractory mutation system pcr
    Human Brafv600e Amplification Refractory Mutation System Pcr, supplied by Amoy Diagnostics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+amplification/braf+detection+fluorescence+gene+human+joint+kit+kras+mutation+nras+pcr+pik3ca/pm42260747-47-7-17
    Average 86 stars, based on 1 article reviews
    human brafv600e amplification refractory mutation system pcr - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    99
    Bio-Rad real time pcr amplification
    Real Time Pcr Amplification, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+amplification/CFX96+Touch/pm42117864-105-0-10
    Average 99 stars, based on 1 article reviews
    real time pcr amplification - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Eppendorf AG pcr amplification instrument
    Pcr Amplification Instrument, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+amplification/Eppendorf/pm42118147-289-6-9
    Average 99 stars, based on 1 article reviews
    pcr amplification instrument - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    96
    TaKaRa smart race cdna amplification kit
    Smart Race Cdna Amplification Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+amplification/SMARTer+PCR+cDNA+Synthesis+Kit/us12624089-385-2-7
    Average 96 stars, based on 1 article reviews
    smart race cdna amplification kit - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    86
    Novoprotein polymerase chain reaction pcr amplification
    Polymerase Chain Reaction Pcr Amplification, supplied by Novoprotein, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+amplification/amplification+chain+pcr+polymerase+reaction/pm42114776-71-5-10
    Average 86 stars, based on 1 article reviews
    polymerase chain reaction pcr amplification - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    96
    TaKaRa takara reverse transcription kit
    Takara Reverse Transcription Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+amplification/PCR+Amplification+Kit/pmc13144552-105-8-8
    Average 96 stars, based on 1 article reviews
    takara reverse transcription kit - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results